Transforming activity of human tumor DNAs.

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Formation of Heterozygotes by Annealing a Mixture of Transforming Dnas.

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APPLICATION OF TWO-DIMENSIONAL ELECTROPHORESIS AND NIH 3T3 CELL TRANSFECTION ASSAY IN THE STUDY OF TUMOR-AS SOCIATED PROTEINS AND GENOMIC DNA TUMOROGENICITY IN MALIGNANT HUMAN ESOPHAGEAL SPECIMENS

Total protein and DNA extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and DNA transforming activity in NIH 3T3 cell transfection assay, respectively. In comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptid...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1981

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.78.2.1181